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KMID : 0361420120360020197
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Journal of Korean Academy of Rehabilitation Medicine
2012 Volume.36 No. 2 p.197 ~ p.206
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The Effect of Human Placental Extract on Rheumatoid Arthritis in an Animal Model
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Park Jeong-Dong
Lee Sang-Il
Kim A-Ram
Park Jong-Moon
Shin Sang-Yeop
Shin Jun-Hwa
Moon Seung-Won
Park Hyun
Oh Min-Kyun
Shin Hee-Suk
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Abstract
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Objective: To assess the effi cacy of human placental extract (HPE) in an animal model of rheumatoid arthritis (RA).
Method: We used (i) KRN C57BL/6 TCR transgenic x NOD mice (KBx/N) serum transfer arthritis and (ii) collageninduced arthritis (CIA) mice to evaluate the effi cacy of HPE (1 ul or 100 ul, intra-peritoneal, three times per week) on RA. Incidence, severity of arthritis, and hind-paw thickness were quantifi ed. Joint destruction was analyzed using modifi ed mammographic imaging. Histopathological analysis for infl ammation, cartilage, and osteoclasts was performed using Hematoxylin-eosin (H-E), safranin-O, and tartrate-resistant acidic phosphatase (TRAP). ELISAs were used for detection of various cytokines in serum and joint tissue.
Results: Th ere were no signifi cant diff erences in incidence of arthritis, clinical scores of arthritis, and hind-paw thickness between HPE-treated and vehicle-treated groups for up to 2 weeks in the KBx/N serum transfer arthritis model. Histopathological analysis also showed no diff erences 2 weeks after treatment. Levels of TNF-?, IL-1?, IL-6, IL-10, and RANKL in serum and joint tissues were similar in all groups. Furthermore, there were no diff erences in clinical, radiological, and histological parameters between HPE-treated and vehicle-treated group for 3 weeks in the CIA model.
Conclusion: Systemic treatment with HPE has no benefi cial eff ects on arthritis in animal models of RA. Th erefore, indiscreet use of HPE in RA should be forbidden.
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KEYWORD
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Human placental extract (HPE), Rheumatoid arthritis (RA)
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